Friday, July 8, 2011

Review and Revise

After meeting Ms Tan, I need to do another batch of bacteria culture as I did not have negative control (an agar plate that has absolutely nothing done to it, to see the sterility of the agar plates), so in this new batch there will be a 7th plate, the negative control. I also need another batch to consolidate the results I have which are right now rather messy. If i have another set of results, I can analyse it more and perhaps identify the outliers more easily.

Another matter I am considering is the method of presenting my data. After all, I really have a lot of data. Right now I'm presenting in a table and maybe graphs the average amount of each type of bacteria on agar inoculated by each agent ON THE 3RD DAY, and on the 3rd day only. I have not yet decided whether to use the data of Days 1 and 2 anywhere. I think I need to decide because if not, I can save work and time by not counting the bacteria on Days 1 and 2 for my 4th batch.

I'm thinking to not waste any data, and present data about the rate with which DIFFERENT TYPES OF BACTERIA appear on plates inoculated with DIFFERENT AGENTS. to this, i can plot a combined graph of each type of bacteria, x-axis the number of days, y-axis the amount of bacteria, different lines representing different agents. Or I can plot combined graphs of each type of agent, with the same axes, the different lines representing the different types of bacteria. It all seems very very complicated.

So should I present the rate at which bacteria appear? It promises to be interesting, but is it relevant to my research question which is about the amount of bacteria removed? Does the rate at which bacteria colonies grow have any significance on the amount of bacteria inoculated onto the plate?

Or - another idea - maybe i can just use the data from Day 1, immediately after inoculation and on 3rd day.

Ms Tan also advise me to:
1, use pi to calculate area
2, use imageJ?
3, think about whether using different fingers affect the amount of bacteria
4, THE OPAQUE WHITE 'MOULD' IS ACTUALLY BACTERIA.
5, use the great photos i have so painstakingly taken!! yay, should i attached ALL of them as appendix?
By the way, is there a limit for appendix pages?

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