I saw Ms Tan just now and she raised some ideas mostly regarding my method which I think is very usable:
1, She suggested that I do not grow bacteria first and then put in the agent as it would be different from the way we usually wash our hands, and too tedious anyway. Instead, I would wash/treat/rinse (haven't figured out exactly how to apply the substance...need to research on the way people use organic substances as antiseptics) one of my finger using one of the substances each time, and then use the cotton swab to swipe from my finger and spread on the bacteria culture. And use one uncleaned finger for bacteria culture to use as control (original amount of bacteria on our hands.)
2, Observe the bacteria growth from the cultures after 24 hours, 48 hours, 72 hours.
3, Need to repeat the experiment a few times for more accurate results.
4, For analysis, compare the samples with each other rather than with the control (before-after). See how natural products compare with commercial products.
5, To measure results correctly, use ImageJ (a software - haven't tried out yet). It can help me calculate the area of bacteria. And the type too? (see colour).
Things to do:
1, arrange lab/equipment usage. (first week of June hols - each time i take observation I can start a new batch, 3 batches, equipment needed - 18 agar jelly sets.)
2, No need to measure pH now i think, or maybe just for lime and lemon to see in which fruit the citric acid present is stronger.
3, Figure out a way to clean and keep fingers unclean that would be fair and leave me with enough fingers. (for the lack of hand area I can't do all 3 batches at one go; but i can try to solve this problem..)
4, Research on how people use/claim to use organic substances to clean their hands.
5, Research on how people use hand soap to clean their hands.
6, Settle on a uniform but fair method.
7, settle research question, hypothesis etc.
8, DO SCIENCE PROPOSAL.
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