Final Experiment Procedure

After the many changes my experimental plan has gone through, I come up with this final procedure. 


Materials and Apparatus: 
18 prepared agar petri dishes 
20 ml of pure lime juice 
20 ml of pure lemon juiceDistilled water
20 ml of normal hand soap (brand:       )
20 ml of antibacterial hand soap that claims to kill 99.9% of bacteria (Dettol brand)
5 sterilized falcon tubes
1 10 ml syringe without needle 
1 timer
Tap waterScotch tape


Pre-experimental preparations:
1, squeeze pure lime juice from Small Limes bought from supermarket, into 1 labeled falcon tube.
2, squeeze pure lemon juice from Green Seedless Lemon bought from supermarket, into 1 labeled falcon tube.
3, squeeze normal hand soap and antibacterial hand soap into respective labeled falcon tubes.
4, test the pH of lime juice and lemon juice for data analysis to see which contains more citric acid.
5, prepare sufficient distilled water obtained from school lab into 1 labeled falcon tube. 
6, cleanse the syringe with distilled water.
7, rinse right thumb and index finger under tap water. 


Procedure:
(Day 1)
1, Prepare 6 lidded agar plates. 
2, Inoculate 1 agar plate with the uncleaned left thumb by streaking in a systematic, even, S shaped pattern across the agar. Use the entire finger and not just the finger tip. Lid and label the plate.
3, Clean the left index finger with lime juice. Use the syringe to extract 5ml of lime juice from the falcon tube and pour evenly onto the finger. Scrub evenly and firmly with right thumb and index finger 10 strokes. Let the lime juice stay on the finger for another 10 seconds. Rinse finger under running water for 10 seconds.
4, Repeat Step 2 with the cleaned left index finger. 
5, Cleanse the syringe with distilled water and right thumb and index finger under tap water. 
6, Clean the left middle finger with lemon juice by repeating Step 3. 
7, Repeat Step 2 with the cleaned left middle finger. 
8, Repeat step 5. 
9, Clean the left ring finger with normal (BRAND) hand soap by repeating step 3. 

10, Repeat Step 2 with the cleaned left ring finger. 

11, Repeat step 5. 

12, Clean the left little finger with Dettol antibacterial hand soap by repeating Step 3. 

13, Repeat Step 2 with the cleaned left little finger. 

14, Repeat step 5. 

15, Clean the right middle finger with distilled water by repeating Step 3. 

16, Repeat Step 2 with the cleaned right middle finger. 

17, Seal all agar plates with Scotch tape and leave for 24 hours in a warm location of about 28 degrees C, out of direct sunlight. 

(Day 2)

18, Repeat Steps 1-17 with another 6 agar plates, using control on RMF, lime juice on LT, lemon juice on LIF, normal hand soap on LMF, antibacterial soap on LRF, distilled water on LLF. 

19, Observe the first 6 plates from Day 1. Record the amount of bacteria growth by organizing the bacteria colonies using a chart of various bacteria colony sizes and counting the number of colonies. Also record the different types of bacteria that grow. Take photographs. 

(Day 3)

20, Repeat steps 1-17 with another 6 plates, using control on LLF, lime juice on RMF, lemon juice on LT, normal hand soap on LIF, antibacterial hand soap on LMF, distilled water on LRF. 

21, Observe bacteria growth on the plates from Days 1 and 2.

(Day 4)

22, Repeat step 19 on plates from Days 1, 2, and 3. 

(Day 5)

23, Repeat step 19 on plates from Days 2 and 3.

(Day 6)

24, repeat step 19 on plate from Day 3. 

25, Organize the data, compare from different plates and analyse.